This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RAT +D122H
catalog :
187426
product information
Catalog Number :
187426
Product Name :
RAT +D122H
article :
doi10.1101/2021.11.29.470343
id28228883
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac Dual
backbone_mutation
backbone_originThermo Fisher (Invitrogen)
backbone_size5238
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2021.11.29.470343v5 for bioRxiv preprint.
growth strain :
Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterPH
sequencing_primer_3GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC
sequencing_primer_5ATTCCGGATTATTCATACCGTCCCACCATCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesNkaa1b
geneAtp1a1
id24211
genbank_ids
NM_012504.1
mutationchanged aspartic acid at 129 for histidine
nameATP1A1
shRNA_sequence
size3072
species
10116
Rattus norvegicus
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PaeI
cloning_site_5XhoI
promoterp10
sequencing_primer_3ACGGACCTTTAATTCAACCCAACACAATATA
sequencing_primer_5CGGGTTCCTTCCGGTATTGTCTCCTTC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATPBS
geneAtp1b1
id25650
genbank_ids
NM_013113.2
mutation
nameATP1B1
shRNA_sequence
size915
species
10116
Rattus norvegicus
tags
resistance markers :
5372
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA