This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CMV-SOX2-T2A-miRFP670
catalog :
187375
citations: 1
Reference
Abatti L, Lado Fern xe1 ndez P, Huynh L, Collado M, Hoffman M, Mitchell J. Epigenetic reprogramming of a distal developmental enhancer cluster drives SOX2 overexpression in breast and lung adenocarcinoma. Nucleic Acids Res. 2023;51:10109-10131 pubmed publisher
product information
Catalog Number :
187375
Product Name :
CMV-SOX2-T2A-miRFP670
article :
doi10.1093/nar/gkad734
id28215608
pubmed_id37738673
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3-TOPO
backbone_mutationInserted T2A-miRFP670 at 3' terminal of MCS.
backbone_originInvitrogen
backbone_size6390
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.
origin :
37
pi :
alt_names
SRY-Box Transcription Factor 2
ANOP3
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3GGATTCTCCTCCACGTCACC
sequencing_primer_5TAGAAGACACCGGGACCGAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
genbank_ids
NM_003106
mutation
nameSOX2
shRNA_sequence
size954
species
9606
Homo sapiens
tags
locationC terminal on insert
tagT2A-miRFP670
plasmid copy :
SOX2 was subcloned from Harvard Institute of Proteomics (HsCD00079917)
resistance markers :
4497
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA