pCMV mouse sidekick2 | Addgene 18735 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV mouse sidekick2

catalog :

18735

citations: 1

Reference
Yamagata M, Sanes J. Dscam and Sidekick proteins direct lamina-specific synaptic connections in vertebrate retina. Nature. 2008;451:465-9 pubmed publisher

product information

Catalog Number :

18735

Product Name :

pCMV mouse sidekick2

article :

doi	10.1038/nature06469
id	2160
pubmed_id	18216854

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-N1 (EGFP deleted)

backbone_mutation

backbone_origin

Clontech

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The NdeI-NotI fragment obtained from mKIAA1514 plasmid was cloned into the XhoI and NotI sites of EGFP-N1, and the XhoI-NdeI fragment was replaced by a cDNA amplified with primers GGCCCTCGAGTTATAAAGCAACTCGCAAAGAGG and CTGTTGTAGGCAGCCACCTCGATC.

origin :

pi :

alt_names

sidekick 2

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	n/a
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	4632412F08Rik, 5330435L01Rik, Sdk-2, mKIAA1514
gene	Sdk2
id	237979

genbank_ids

mutation

name

Sidekick-2

shRNA_sequence

size

species

10090

Mus musculus