pLL5.0-EGFP-HRasC20 | Addgene 187252 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLL5.0-EGFP-HRasC20

catalog :

187252

citations: 1

Reference
Katsuno Kambe H, Teo J, Ju R, Hudson J, Stehbens S, Yap A. Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation. elife. 2021;10: pubmed publisher

product information

Catalog Number :

187252

Product Name :

pLL5.0-EGFP-HRasC20

article :

doi	10.7554/eLife.67915
id	28225773
pubmed_id	34661524

bacterial resistance :

Ampicillin

cloning :

backbone

pLL5.0

backbone_mutation

backbone_origin

backbone_size

6919

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

pLL5.0-EGFP was digested with EcoRI and SbfI to remove EGFP, and then EGFP-C20 fragment was inserted and ligated by In-Fusion cloning kit. 5' cloning site: EcoR1 (destroyed), 3' cloning site: Sbf1 (destroyed).

growth strain :

Lenticiral expression of EGFP-HRasC20

origin :

pi :

alt_names

CAAX

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	5' LTR
sequencing_primer_3
sequencing_primer_5	EGFP, forward primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

name

HRasC20

shRNA_sequence

size

795

species

9606

Homo sapiens