This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-Tau(T217A)
catalog :
187025
citations: 1
Reference
Rajbanshi B, Guruacharya A, Mandell J, Bloom G. Localization, induction, and cellular effects of tau phosphorylated at threonine 217. Alzheimers Dement. 2023;19:2874-2887 pubmed publisher
product information
Catalog Number :
187025
Product Name :
EGFP-Tau(T217A)
article :
doi10.1002/alz.12892
id28228809
pubmed_id36633254
bacterial resistance :
Ampicillin
cloning :
backbonepRK5
backbone_mutation
backbone_originClontech
backbone_size5370
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://doi.org/10.1101/2022.04.18.488657 for bioRxiv preprint.
growth strain :
Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter
origin :
37
pi :
alt_names
Tau
This T217A tau construct contains human four-repeat tau and two inserts in N-terminal sequences with point mutation at T217A
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40
geneMAPT
id4137
genbank_ids
mutationChanged Threonine 217 to Alanine
nameMAPT
shRNA_sequence
size2055
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
6070
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA