This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28b-MBP-TEV-PWWP_DNMT3A
catalog :
186970
product information
Catalog Number :
186970
Product Name :
pET28b-MBP-TEV-PWWP_DNMT3A
article :
doi
id28228802
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepET28b
backbone_mutation
backbone_origin
backbone_size5222
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of human DNMT3A PWWP domain (residues 278 427) with His-MBP tag and TEV protease cleavage site.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XbaI
promoterT7
sequencing_primer_3T7-SR GCTAGTTATTGCTCAGCGG
sequencing_primer_5pET-SF CAGCAACCGCACCTGTGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDNMT3A2, HESJAS, M.HsaIIIA, TBRS
geneDNMT3A
id1788
genbank_ids
NM_022552.4
mutationPWWP domain (residues 278 427)
nameDNMT3A PWWP domain
shRNA_sequence
size1750
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagHis-MBP
plasmid copy :
The His-MBP-TEV insert was derived from MBP-FUS_FL_WT, a gift from N. Fawzi (Addgene #98651).
resistance markers :
6033
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA