This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCF806-shABC2
catalog :
186714
citations: 1
Reference
Amen A, Loughran R, Huang C, Lew R, Ravi A, Guan Y, et al. Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi. Cell Rep Methods. 2022;2:100239 pubmed publisher
product information
Catalog Number :
186714
Product Name :
pCF806-shABC2
article :
doi10.1016/j.crmeth.2022.100239
id28228766
pubmed_id35880017
bacterial resistance :
Ampicillin
cloning :
backbonepCF806
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth strain :
Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
aliasesA-RAF, ARAF1, PKS2, RAFA1
geneARAF
id369
genbank_ids
mutation
nameARAF, BRAF, CRAF
shRNA_sequencettagattttgtcaagatgggct, tttctgtaaggctttcacgtta, ttaaacaattcttaaacctgag
size
species
9606
Homo sapiens
tags
resistance markers :
6028
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA