This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mEGFP-HRas
catalog :
18662
citations: 16
Reference
Gupta A, Lu D, Balasubramanian H, Chi Z, Wohland T. Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes. J Lipid Res. 2022;63:100220 pubmed publisher
Tejeda Muñoz N, Morselli M, Moriyama Y, Sheladiya P, Pellegrini M, De Robertis E. Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosis. iScience. 2022;25:104123 pubmed publisher
Chytła A, Gajdzik Nowak W, Biernatowska A, Sikorski A, Czogalla A. High-Level Expression of Palmitoylated MPP1 Recombinant Protein in Mammalian Cells. Membranes (Basel). 2021;11: pubmed publisher
Katoch A, Jamwal V, Faheem M, Kumar S, Senapati S, Yadav G, et al. Overlapping targets exist between the Par-4 and miR-200c axis which regulate EMT and proliferation of pancreatic cancer cells. Transl Oncol. 2021;14:100879 pubmed publisher
Motamedi N, Sewald X, Luo Y, Mothes W, DiMaio D. SV40 Polyomavirus Activates the Ras-MAPK Signaling Pathway for Vacuolization, Cell Death, and Virus Release. Viruses. 2020;12: pubmed publisher
Redchuk T, Karasev M, Verkhusha P, Donnelly S, Hülsemann M, Virtanen J, et al. Optogenetic regulation of endogenous proteins. Nat Commun. 2020;11:605 pubmed publisher
Podkalicka J, Biernatowska A, Olszewska P, Tabaczar S, Sikorski A. The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras. Oncotarget. 2018;9:18410-18421 pubmed publisher
Luo X, Li C, Tan R, Xu X, Wu W, Satoh A, et al. A RasGAP, DAB2IP, regulates lipid droplet homeostasis by serving as GAP toward RAB40C. Oncotarget. 2017;8:85415-85427 pubmed publisher
Ng M, Wang M, Casey P, Gan Y, Hagen T. Activation of MAPK/ERK signaling by Burkholderia pseudomallei cycle inhibiting factor (Cif). PLoS ONE. 2017;12:e0171464 pubmed publisher
Mattison S, Blatch G, Edkins A. HOP expression is regulated by p53 and RAS and characteristic of a cancer gene signature. Cell Stress Chaperones. 2017;22:213-223 pubmed publisher
Di Rienzo C, Cardarelli F, Di Luca M, Beltram F, Gratton E. Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells. Biophys J. 2016;111:841-851 pubmed publisher
Frei D, Hodneland E, Rios Mondragon I, Burtey A, Neumann B, Bulkescher J, et al. Novel microscopy-based screening method reveals regulators of contact-dependent intercellular transfer. Sci Rep. 2015;5:12879 pubmed publisher
Wigerius M, Asghar N, Melik W, Johansson M. Scribble controls NGF-mediated neurite outgrowth in PC12 cells. Eur J Cell Biol. 2013;92:213-21 pubmed publisher
Kong E, Peng S, Chandra G, Sarkar C, Zhang Z, Bagh M, et al. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43. J Biol Chem. 2013;288:9112-25 pubmed publisher
Wurtzel J, Kumar P, Goldfinger L. Palmitoylation regulates vesicular trafficking of R-Ras to membrane ruffles and effects on ruffling and cell spreading. Small Gtpases. 2012;3:139-53 pubmed publisher
Yasuda R, Harvey C, Zhong H, Sobczyk A, Van Aelst L, Svoboda K. Supersensitive Ras activation in dendrites and spines revealed by two-photon fluorescence lifetime imaging. Nat Neurosci. 2006;9:283-91 pubmed
product information
Catalog Number :
18662
Product Name :
mEGFP-HRas
article :
doi10.1038/nn1635
id2126
pubmed_id16429133
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutation
backbone_originPromega
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
HRas
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EBV-rev
sequencing_primer_5EGFP-C; chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutation
nameH-Ras
shRNA_sequence
size600
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmonomeric EGFP
plasmid copy :
M. Matsuda (Osaka University)
resistance markers :
511
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA