This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FKBP-AP180-GFP
catalog :
186577
citations: 1
Reference
K xfc ey C, Sittewelle M, Larocque G, Hern xe1 ndez Gonz xe1 lez M, Royle S. Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. elife. 2022;11: pubmed publisher
product information
Catalog Number :
186577
Product Name :
FKBP-AP180-GFP
article :
doi10.7554/eLife.78929
id28228740
pubmed_id35852853
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note: The plasmid is difficult to sequence near bases 2140-2220 that are within the insert.
growth strain :
Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.
origin :
37
pi :
alt_names
SNAP91
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pCMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAp180
geneSnap91
id65178
genbank_ids
mutation
nameAP180
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
location
tagFKBP N-terminal GFP C-terminal
resistance markers :
2079
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA