pET-EIN-FTO(32-505) | Addgene 186466 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-EIN-FTO(32-505)

catalog :

186466

citations: 1

Reference
Khatiwada B, Purslow J, Underbakke E, Venditti V. N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5. Protein Expr Purif. 2019;167:105540 pubmed publisher

product information

Catalog Number :

186466

Product Name :

pET-EIN-FTO(32-505)

article :

doi	10.1016/j.pep.2019.105540
id	28203281
pubmed_id	31740367

bacterial resistance :

Ampicillin

cloning :

backbone

pET (21a)

backbone_mutation

The size is 6Histidine+EIN+ Pet21a

backbone_origin

Novagen

backbone_size

6222

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The article where this gene was cloned is as follows- https://pubmed.ncbi.nlm.nih.gov/31740367/ This gene is similar to another addgene deposit (Number 125732) instead of Alkbh5 sequence FTO sequence is inserted.

growth strain :

Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

origin :

pi :

alt_names

FTO

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3
sequencing_primer_5	ATTTCAGGCATTTTAGCAT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALKBH9, BMIQ14, GDFD, IFEX9
gene	FTO
id	79068

genbank_ids

mutation

amino acid 32-505

name

Fat mass obesity associated protein

shRNA_sequence

size

1421

species

9606

Homo sapiens