This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
catalog :
186413
citations: 1
Reference
Roure A, Rothbächer U, Robin F, Kalmar E, Ferone G, Lamy C, et al. A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. PLoS ONE. 2007;2:e916 pubmed
product information
Catalog Number :
186413
Product Name :
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
article :
doi10.1371/journal.pone.0000916
id3089
pubmed_id17878951
bacterial resistance :
Chloramphenicol and Ampicillin
cloning :
backbonepDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
backbone_mutation
backbone_originPMID: 17878951
backbone_size5298
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway destination vector
growth notes :
Gateway destination vector for electroporation experiments.
growth strain :
Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3Rv_pSP172BSSPE: TTGACACCAGACCAACTGGTAATG, SK primer
sequencing_primer_5Fw_pSP172BSSPE: ACAGCTTGTCTGTAAGCGGATGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0344, ECK0341
genelacZ
id945006
genbank_ids
mutation
namelacZ
shRNA_sequence
size
species
32644
Other
E. coli
tags
locationN terminal on insert
tagNuclear localization signal
resistance markers :
4790
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA