This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pENT-L1-Kozak-EGFP-GPI-stop-L2
catalog :
186355
citations: 1
Reference
Roure A, Rothbächer U, Robin F, Kalmar E, Ferone G, Lamy C, et al. A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. PLoS ONE. 2007;2:e916 pubmed
product information
Catalog Number :
186355
Product Name :
pENT-L1-Kozak-EGFP-GPI-stop-L2
article :
doi10.1371/journal.pone.0000916
id3089
pubmed_id17878951
bacterial resistance :
Kanamycin
cloning :
backbonePDONR-221 P1-P2
backbone_mutation
backbone_originPMID: 14736459
backbone_size2544
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Expression of a fluorescent membrane marker
growth strain :
Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13rev: caggaaacagctatgac
sequencing_primer_5M13forward: TGTAAAACGACGGCCAGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliases16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20
geneCD59
id966
genbank_ids
mutation
namesignal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site
shRNA_sequence
size936
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagEGFP
resistance markers :
4790
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA