This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCSG28-SH3-588
catalog :
185920
citations: 1
Reference
Park R, Ongpipattanakul C, Nair S, Bowers A, Kuhlman B. Designer installation of a substrate recruitment domain to tailor enzyme specificity. Nat Chem Biol. 2022;: pubmed publisher
product information
Catalog Number :
185920
Product Name :
pMCSG28-SH3-588
article :
doi10.1038/s41589-022-01206-0
id28228618
pubmed_id36509904
bacterial resistance :
Ampicillin
cloning :
backbonepMCSG28
backbone_mutationInsertion between RBS and TEV/6x HIS site
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationDesigned enzyme with novel interface between humanized rat COMT and the human Fyn SH3 domain (numerous mutations)
nameSH3-588
shRNA_sequence
size900
species
100
Synthetic
tags
locationC terminal on insert
tag6x HIS tag
resistance markers :
1351
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA