This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Raptor_2 shRNA
catalog :
1858
citations: 16
Reference
Kaur H, Moreau R. Raptor knockdown concurrently increases the electrical resistance and paracellular permeability of Caco-2 cell monolayers. Life Sci. 2022;308:120989 pubmed publisher
He B, Wang Z, Moreau R. Chylomicron production is repressed by RPTOR knockdown, R-α-lipoic acid and 4-phenylbutyric acid in human enterocyte-like Caco-2 cells. J Nutr Biochem. 2022;108:109087 pubmed publisher
Conlon M, Poltorack C, Forcina G, Armenta D, Mallais M, Perez M, et al. A compendium of kinetic modulatory profiles identifies ferroptosis regulators. Nat Chem Biol. 2021;17:665-674 pubmed publisher
Kaur H, Moreau R. Curcumin represses mTORC1 signaling in Caco-2 cells by a two-sided mechanism involving the loss of IRS-1 and activation of AMPK. Cell Signal. 2021;78:109842 pubmed publisher
He B, Moreau R. R-α-Lipoic Acid and 4-Phenylbutyric Acid Have Distinct Hypolipidemic Mechanisms in Hepatic Cells. Biomedicines. 2020;8: pubmed publisher
Fan S, Kroeger B, Marie P, Bridges E, Mason J, McCormick K, et al. Glutamine deprivation alters the origin and function of cancer cell exosomes. EMBO J. 2020;39:e103009 pubmed publisher
Sun H, Yang W, Tian Y, Zeng X, Zhou J, Mok M, et al. An inflammatory-CCRK circuitry drives mTORC1-dependent metabolic and immunosuppressive reprogramming in obesity-associated hepatocellular carcinoma. Nat Commun. 2018;9:5214 pubmed publisher
Kaur H, He B, Zhang C, Rodriguez E, Hage D, Moreau R. Piperine potentiates curcumin-mediated repression of mTORC1 signaling in human intestinal epithelial cells: implications for the inhibition of protein synthesis and TNF? signaling. J Nutr Biochem. 2018;57:276-286 pubmed publisher
Pu J, Keren Kaplan T, Bonifacino J. A Ragulator-BORC interaction controls lysosome positioning in response to amino acid availability. J Cell Biol. 2017;216:4183-4197 pubmed publisher
Hori I, Otomo T, Nakashima M, Miya F, Negishi Y, Shiraishi H, et al. Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Sci Rep. 2017;7:3552 pubmed publisher
Cao J, Tyburczy M, Moss J, Darling T, Widlund H, Kwiatkowski D. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest. 2017;127:349-364 pubmed publisher
Kim E, Kim A, Kim S, Kim H, Chang J, Ahn C, et al. Inhibition of mTORC1 induces loss of E-cadherin through AKT/GSK-3? signaling-mediated upregulation of E-cadherin repressor complexes in non-small cell lung cancer cells. Respir Res. 2014;15:26 pubmed publisher
Wang C, Qin L, Manes T, Kirkiles Smith N, Tellides G, Pober J. Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. J Exp Med. 2014;211:395-404 pubmed publisher
Peña Llopis S, Vega Rubin de Celis S, Schwartz J, Wolff N, Tran T, Zou L, et al. Regulation of TFEB and V-ATPases by mTORC1. EMBO J. 2011;30:3242-58 pubmed publisher
Gwinn D, Asara J, Shaw R. Raptor is phosphorylated by cdc2 during mitosis. PLoS ONE. 2010;5:e9197 pubmed publisher
Sarbassov D, Guertin D, Ali S, Sabatini D. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098-101 pubmed
product information
Catalog Number :
1858
Product Name :
Raptor_2 shRNA
article :
doi10.1126/science.1106148
id132
pubmed_id15718470
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_originStewart SA, RNA 2003 Apr; 9(4):493-501.
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
This plasmid is referred to as Raptor shRNA_1 in the science article. Sense: 5
growth temp :
XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
inserts :
Unknown
origin :
37
pi :
alt_names
275 Raptor_2
shRNA Raptor
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesKOG1, Mip1
geneRPTOR
id57521
genbank_ids
mutation
nameRaptor
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA