This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKL100
catalog :
185708
citations: 1
Reference
Leng K, Rose I, Kim H, Xia W, Romero Fernandez W, Rooney B, et al. CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states. Nat Neurosci. 2022;25:1528-1542 pubmed publisher
product information
Catalog Number :
185708
Product Name :
pKL100
article :
doi10.1038/s41593-022-01180-9
id28225286
pubmed_id36303069
bacterial resistance :
Ampicillin
cloning :
backbonepUCM
backbone_mutation
backbone_origin
backbone_size10600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
AAVS1 safe-harbor integration of dox-inducible NFIA-SOX9
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5AflII
promoterTRE3G
sequencing_primer_3GAAATTTGTGATGCTATTGC
sequencing_primer_5AGCTCGTTTAGTGAACCGTCAGATC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRMUTD, CTF, NF-I/A, NF1-A, NFI-A, NFI-L
geneNFIA
id4774
aliasesCMD1, CMPD1, SRA1, SRXX2, SRXY10
geneSOX9
id6662
genbank_ids
ENST00000403491
ENST00000245479
mutation
nameNFIA-SOX9
shRNA_sequence
size3100
species
9606
Homo sapiens
tags
resistance markers :
3107
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA