Raptor_1 shRNA | Addgene 1857 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Raptor_1 shRNA

catalog :

1857

citations: 15

Reference
Majeed S, Majeed R, Malik A, Andrabi K. MTORC2 is a physiological hydrophobic motif kinase of S6 Kinase 1. Biochim Biophys Acta Mol Cell Res. 2023;1870:119449 pubmed publisher
Collins S, Wiegand M, Werner A, Brown I, Mundo M, Swango D, et al. Ras-mediated activation of mTORC2 promotes breast epithelial cell migration and invasion. Mol Biol Cell. 2023;34:ar9 pubmed publisher
Kong B, Min S, Lee C, Cho Y. Mitochondrial-encoded MOTS-c prevents pancreatic islet destruction in autoimmune diabetes. Cell Rep. 2021;36:109447 pubmed publisher
Majeed S, Batool A, Majeed R, Bhat N, Zargar M, Andrabi K. mTORC1 induces eukaryotic translation initiation factor 4E interaction with TOS-S6 kinase 1 and its activation. Cell Cycle. 2021;20:839-854 pubmed publisher
Liu W, Yang H, Hsu C, Wang C, Wang T, Kao J, et al. Pegylated IFN-Î± suppresses hepatitis C virus by promoting the DAPK-mTOR pathway. Proc Natl Acad Sci U S A. 2016;113:14799-14804 pubmed publisher
Faes S, Duval A, Planche A, Uldry E, Santoro T, Pythoud C, et al. Acidic tumor microenvironment abrogates the efficacy of mTORC1 inhibitors. Mol Cancer. 2016;15:78 pubmed
Gandin V, Masvidal L, Cargnello M, Gyenis L, McLaughlan S, Cai Y, et al. mTORC1 and CK2 coordinate ternary and eIF4F complex assembly. Nat Commun. 2016;7:11127 pubmed publisher
Bailey S, Zhou B, Damrauer J, Krishnan B, Wilson H, Smith A, et al. mTOR inhibition induces compensatory, therapeutically targetable MEK activation in renal cell carcinoma. PLoS ONE. 2014;9:e104413 pubmed publisher
Chang W, Wei K, Ho L, Berry G, Jacobs S, Chang C, et al. A critical role for the mTORC2 pathway in lung fibrosis. PLoS ONE. 2014;9:e106155 pubmed publisher
Selvaraj N, Budka J, Ferris M, Jerde T, Hollenhorst P. Prostate cancer ETS rearrangements switch a cell migration gene expression program from RAS/ERK to PI3K/AKT regulation. Mol Cancer. 2014;13:61 pubmed publisher
Kim E, Kim A, Kim S, Kim H, Chang J, Ahn C, et al. Inhibition of mTORC1 induces loss of E-cadherin through AKT/GSK-3? signaling-mediated upregulation of E-cadherin repressor complexes in non-small cell lung cancer cells. Respir Res. 2014;15:26 pubmed publisher
Malek M, Guillaumot P, Huber A, Lebeau J, Petrilli V, Kfoury A, et al. LAMTOR1 depletion induces p53-dependent apoptosis via aberrant lysosomal activation. Cell Death Dis. 2012;3:e300 pubmed publisher
Copp J, Manning G, Hunter T. TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2. Cancer Res. 2009;69:1821-7 pubmed publisher
Ramírez Valle F, Braunstein S, Zavadil J, Formenti S, Schneider R. eIF4GI links nutrient sensing by mTOR to cell proliferation and inhibition of autophagy. J Cell Biol. 2008;181:293-307 pubmed publisher
Sarbassov D, Guertin D, Ali S, Sabatini D. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098-101 pubmed

product information

Catalog Number :

1857

Product Name :

Raptor_1 shRNA

article :

doi	10.1126/science.1106148
id	132
pubmed_id	15718470

bacterial resistance :

Ampicillin

cloning :

backbone

pLKO.1

backbone_mutation

backbone_origin

Stewart SA, RNA 2003 Apr; 9(4):493-501.

backbone_size

7032

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

This plasmid is referred to as Raptor shRNA_2 in the science article. Sense: CCGGAGGGCC CTGCTACTCG CTTTTCTCGA GAAAAGCGAG TAGCAGGGCC CTTTTTTG. Antisense: AATTCAAAAA AGGGCCCTGC TACTCGCTTT TCTCGAGAAA AGCGAGTAGC AGGGCCC. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

growth temp :

XL10-Gold Ultracompetent Cells from Stratagene. 37oC.

inserts :

XL10-Gold

origin :

pi :

alt_names

65 Raptor_1

shRNA Raptor

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	AgeI
promoter
sequencing_primer_3
sequencing_primer_5	LKO.1 5'
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	KOG1, Mip1
gene	RPTOR
id	57521

genbank_ids

mutation

name

Raptor

shRNA_sequence

size

species

9606

Homo sapiens