This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mTOR_2 shRNA
catalog :
1856
citations: 5
Reference
Zhan H, Bhattacharya S, Cai H, Iglesias P, Huang C, Devreotes P. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. Dev Cell. 2020;54:608-623.e5 pubmed publisher
Wang P, Xie R, Cheng M, Sapolsky R, Ji X, Zhao H. The mTOR cell signaling pathway is crucial to the long-term protective effects of ischemic postconditioning against stroke. Neurosci Lett. 2018;676:58-65 pubmed publisher
Wang C, Qin L, Manes T, Kirkiles Smith N, Tellides G, Pober J. Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. J Exp Med. 2014;211:395-404 pubmed publisher
Krajcovic M, Krishna S, Akkari L, Joyce J, Overholtzer M. mTOR regulates phagosome and entotic vacuole fission. Mol Biol Cell. 2013;24:3736-45 pubmed publisher
Sarbassov D, Guertin D, Ali S, Sabatini D. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098-101 pubmed
product information
Catalog Number :
1856
Product Name :
mTOR_2 shRNA
article :
doi10.1126/science.1106148
id132
pubmed_id15718470
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_originStewart SA, RNA 2003 Apr; 9(4):493-501.
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
This plasmid is referred to as mTOR_1 in the science paper. Sense: 5
growth temp :
XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
origin :
37
pi :
alt_names
RAFT1
193 mTOR_2
shRNA mTOR
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutation
namemTOR
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA