This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mTOR_1 shRNA
catalog :
1855
citations: 11
Reference
Majeed S, Batool A, Majeed R, Bhat N, Zargar M, Andrabi K. mTORC1 induces eukaryotic translation initiation factor 4E interaction with TOS-S6 kinase 1 and its activation. Cell Cycle. 2021;20:839-854 pubmed publisher
Zhan H, Bhattacharya S, Cai H, Iglesias P, Huang C, Devreotes P. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. Dev Cell. 2020;54:608-623.e5 pubmed publisher
Okada K, Nogami A, Ishida S, Akiyama H, Chen C, Umezawa Y, et al. FLT3-ITD induces expression of Pim kinases through STAT5 to confer resistance to the PI3K/Akt pathway inhibitors on leukemic cells by enhancing the mTORC1/Mcl-1 pathway. Oncotarget. 2018;9:8870-8886 pubmed publisher
Button R, Roberts S, Willis T, Hanemann C, Luo S. Accumulation of autophagosomes confers cytotoxicity. J Biol Chem. 2017;292:13599-13614 pubmed publisher
Erbil S, Oral O, Mitou G, Kig C, Durmaz Timucin E, Guven Maiorov E, et al. RACK1 Is an Interaction Partner of ATG5 and a Novel Regulator of Autophagy. J Biol Chem. 2016;291:16753-65 pubmed publisher
Garros Regulez L, Aldaz P, Arrizabalaga O, Moncho Amor V, Carrasco Garcia E, Manterola L, et al. mTOR inhibition decreases SOX2-SOX9 mediated glioma stem cell activity and temozolomide resistance. Expert Opin Ther Targets. 2016;20:393-405 pubmed publisher
Zeng M, van der Donk W, Chen J. Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Mol Biol Cell. 2014;25:3954-61 pubmed publisher
Selvaraj N, Budka J, Ferris M, Jerde T, Hollenhorst P. Prostate cancer ETS rearrangements switch a cell migration gene expression program from RAS/ERK to PI3K/AKT regulation. Mol Cancer. 2014;13:61 pubmed publisher
Krajcovic M, Krishna S, Akkari L, Joyce J, Overholtzer M. mTOR regulates phagosome and entotic vacuole fission. Mol Biol Cell. 2013;24:3736-45 pubmed publisher
Copp J, Manning G, Hunter T. TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2. Cancer Res. 2009;69:1821-7 pubmed publisher
Sarbassov D, Guertin D, Ali S, Sabatini D. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098-101 pubmed
product information
Catalog Number :
1855
Product Name :
mTOR_1 shRNA
article :
doi10.1126/science.1106148
id132
pubmed_id15718470
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_originStewart SA, RNA 2003 Apr; 9(4):493-501.
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
This plasmid is referred to as mTOR shRNA_2 in the article. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).
growth temp :
XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
inserts :
Unknown
origin :
37
pi :
alt_names
RAFT1
B109 mTOR_1
shRNA mTOR
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutation
namemTOR
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA