This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2+ Zf GFP-vcla
catalog :
185410
citations: 1
Reference
Boutillon A, Escot S, Elouin A, Jahn D, González Tirado S, Starruß J, et al. Guidance by followers ensures long-range coordination of cell migration through α-catenin mechanoperception. Dev Cell. 2022;57:1529-1544.e5 pubmed publisher
product information
Catalog Number :
185410
Product Name :
pCS2+ Zf GFP-vcla
article :
doi10.1016/j.devcel.2022.05.001
id28225222
pubmed_id35613615
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
In vitro synthesis of mRNA
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2021.04.26.441407v2 for bioRxiv preprint.
growth strain :
GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV
origin :
37
pi :
alt_names
vcla, vcl
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseshm:zehn2160, im:6902790, vcl
genevcla
id552980
genbank_ids
mutation
namevinculin a
shRNA_sequence
size3945
species
7955
Danio rerio
tags
locationN terminal on backbone
tageGFP
plasmid copy :
Dr Johan de Rooij, University Medical Center Utrecht
resistance markers :
5965
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA