This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-6P-1-gp78 RING
catalog :
185353
citations: 1
Reference
Smith C, Tsai Y, Liang Y, Khago D, Mariano J, Li J, et al. A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2 and is essential for ER-associated degradation. PLoS Biol. 2021;19:e3001474 pubmed publisher
product information
Catalog Number :
185353
Product Name :
pGEX-6P-1-gp78 RING
article :
doi10.1371/journal.pbio.3001474
id28224682
pubmed_id34879065
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-6P-1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Plasmid contains sequence encoding the entire C-terminus of gp78. Stop codon placed following the RING domain to limit expression to GST-RING domain fusion.
growth strain :
RING domain-containing GST fusion protein for ubiquitination assays.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5-
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGP78, RNF45
geneAMFR
id267
genbank_ids
mutationStop codon after aa 393
namegp78 cytoplasmic domain
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
571
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA