This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Rictor_1 shRNA
catalog :
1853
citations: 23
Reference
Bu C, Zhao L, Wang L, Yu Z, Zhou J. mTORC2 promotes pancreatic cancer progression and parp inhibitor resistance. Oncol Res. 2023;31:495-503 pubmed publisher
Majeed S, Majeed R, Malik A, Andrabi K. MTORC2 is a physiological hydrophobic motif kinase of S6 Kinase 1. Biochim Biophys Acta Mol Cell Res. 2023;1870:119449 pubmed publisher
Zhou H, Guan Q, Hou X, Liu L, Zhou L, Li W, et al. Epithelial-mesenchymal reprogramming by KLF4-regulated Rictor expression contributes to metastasis of non-small cell lung cancer cells. Int J Biol Sci. 2022;18:4869-4883 pubmed publisher
Kong B, Min S, Lee C, Cho Y. Mitochondrial-encoded MOTS-c prevents pancreatic islet destruction in autoimmune diabetes. Cell Rep. 2021;36:109447 pubmed publisher
Conlon M, Poltorack C, Forcina G, Armenta D, Mallais M, Perez M, et al. A compendium of kinetic modulatory profiles identifies ferroptosis regulators. Nat Chem Biol. 2021;17:665-674 pubmed publisher
Zhan H, Bhattacharya S, Cai H, Iglesias P, Huang C, Devreotes P. An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells. Dev Cell. 2020;54:608-623.e5 pubmed publisher
Ozturk D, Kocak M, Akcay A, Kınoglu K, Kara E, Buyuk Y, et al. MITF-MIR211 axis is a novel autophagy amplifier system during cellular stress. Autophagy. 2018;:1-16 pubmed publisher
Chiang C, Demetriou A, Ung N, Choudhury N, Ghaffarian K, Ruderman D, et al. mTORC2 contributes to the metabolic reprogramming in EGFR tyrosine-kinase inhibitor resistant cells in non-small cell lung cancer. Cancer Lett. 2018;434:152-159 pubmed publisher
Maiti S, Mondal S, Satyavarapu E, Mandal C. mTORC2 regulates hedgehog pathway activity by promoting stability to Gli2 protein and its nuclear translocation. Cell Death Dis. 2017;8:e2926 pubmed publisher
Gu Y, Albuquerque C, Braas D, Zhang W, Villa G, Bi J, et al. mTORC2 Regulates Amino Acid Metabolism in Cancer by Phosphorylation of the Cystine-Glutamate Antiporter xCT. Mol Cell. 2017;67:128-138.e7 pubmed publisher
Hori I, Otomo T, Nakashima M, Miya F, Negishi Y, Shiraishi H, et al. Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Sci Rep. 2017;7:3552 pubmed publisher
Liu W, Yang H, Hsu C, Wang C, Wang T, Kao J, et al. Pegylated IFN-α suppresses hepatitis C virus by promoting the DAPK-mTOR pathway. Proc Natl Acad Sci U S A. 2016;113:14799-14804 pubmed publisher
Cao J, Tyburczy M, Moss J, Darling T, Widlund H, Kwiatkowski D. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest. 2017;127:349-364 pubmed publisher
Zeng M, van der Donk W, Chen J. Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Mol Biol Cell. 2014;25:3954-61 pubmed publisher
Bailey S, Zhou B, Damrauer J, Krishnan B, Wilson H, Smith A, et al. mTOR inhibition induces compensatory, therapeutically targetable MEK activation in renal cell carcinoma. PLoS ONE. 2014;9:e104413 pubmed publisher
Chang W, Wei K, Ho L, Berry G, Jacobs S, Chang C, et al. A critical role for the mTORC2 pathway in lung fibrosis. PLoS ONE. 2014;9:e106155 pubmed publisher
Selvaraj N, Budka J, Ferris M, Jerde T, Hollenhorst P. Prostate cancer ETS rearrangements switch a cell migration gene expression program from RAS/ERK to PI3K/AKT regulation. Mol Cancer. 2014;13:61 pubmed publisher
Kim E, Kim A, Kim S, Kim H, Chang J, Ahn C, et al. Inhibition of mTORC1 induces loss of E-cadherin through AKT/GSK-3? signaling-mediated upregulation of E-cadherin repressor complexes in non-small cell lung cancer cells. Respir Res. 2014;15:26 pubmed publisher
Wang C, Qin L, Manes T, Kirkiles Smith N, Tellides G, Pober J. Rapamycin antagonizes TNF induction of VCAM-1 on endothelial cells by inhibiting mTORC2. J Exp Med. 2014;211:395-404 pubmed publisher
Tandon M, Chen Z, Pratap J. Runx2 activates PI3K/Akt signaling via mTORC2 regulation in invasive breast cancer cells. Breast Cancer Res. 2014;16:R16 pubmed publisher
Malek M, Guillaumot P, Huber A, Lebeau J, Petrilli V, Kfoury A, et al. LAMTOR1 depletion induces p53-dependent apoptosis via aberrant lysosomal activation. Cell Death Dis. 2012;3:e300 pubmed publisher
Copp J, Manning G, Hunter T. TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2. Cancer Res. 2009;69:1821-7 pubmed publisher
Sarbassov D, Guertin D, Ali S, Sabatini D. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005;307:1098-101 pubmed
product information
Catalog Number :
1853
Product Name :
Rictor_1 shRNA
article :
doi10.1126/science.1106148
id132
pubmed_id15718470
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_originStewart SA, RNA 2003 Apr; 9(4):493-501.
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
This plasmid is referred to as Rictor_2 in the science paper. Sense: CCGGTACTTG TGAAGAATCG TATCTTCTCG AGAAGATACG ATTCTTCACA AGTTTTTTG. Antisense: AATTCAAAAA ACTTGTGAAG AATCGTATCT TCTCGAGAAG ATACGATTCT TCACAAGTA. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).
growth temp :
XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
origin :
37
pi :
alt_names
shRNA Rictor
25B Rictor_1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAVO3, PIA, hAVO3
geneRICTOR
id253260
genbank_ids
NM_152756
mutation
nameRictor
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA