This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3_mScarlet-LAMP1
catalog :
185138
product information
Catalog Number :
185138
Product Name :
pcDNA3_mScarlet-LAMP1
article :
doi10.5281/zenodo.6363342
id28225214
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originThermo Fisher Scientific
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that this plasmid will send mScarlet to the lumenal side of the lysosome (LAMP1 N terminus) so that it can be used for lysosomal pH sensing. This plasmid is described in a published paper that does not have a PubMed ID. Please cite as DOI: https://doi.org/10.5281/zenodo.6363342
growth strain :
Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime
origin :
37
pi :
alt_names
LAMP1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLGP120
geneLamp1
id25328
genbank_ids
mutationdeleted amino acids 1-21 of LAMP1
nameLysosome-associated membrane glycoprotein 1
shRNA_sequence
size1965
species
10116
Rattus norvegicus
100
Synthetic
tags
locationN terminal on insert
tagmScarlet
resistance markers :
5837
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA