This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
catalog :
184563
citations: 1
Reference
Valenstein M, Rogala K, Lalgudi P, Brignole E, Gu X, Saxton R, et al. Structure of the nutrient-sensing hub GATOR2. Nature. 2022;607:610-616 pubmed publisher
product information
Catalog Number :
184563
Product Name :
K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
article :
doi10.1038/s41586-022-04939-z
id28225111
pubmed_id35831510
bacterial resistance :
Ampicillin
cloning :
backbonepLJM60 (based on pLKO.1 and Lentihair)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.
origin :
30
pi :
alt_names
NPR3 like, GATOR1 complex subunit
Nitrogen permease regulator 3-like protein
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SalI
promoterCMV
sequencing_primer_3TAGTTTGTATGTCTGTTGCTATTA
sequencing_primer_5(1) CMV Forward, (2) SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11
geneNPRL3
id8131
genbank_ids
NM_001077350
mutationAll lysines were mutated to arginines. Codon-optimized for expression in human cells.
nameC16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11
shRNA_sequence
size1710
species
9606
Homo sapiens
tags
resistance markers :
5903
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA