This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV10-3xFLAG-prs-MCP1
catalog :
184341
citations: 1
Reference
Adlakha J, Hong Z, Li P, Reinisch K. Structural and biochemical insights into lipid transport by VPS13 proteins. J Cell Biol. 2022;221: pubmed publisher
product information
Catalog Number :
184341
Product Name :
pCMV10-3xFLAG-prs-MCP1
article :
doi10.1083/jcb.202202030
id28225067
pubmed_id35357422
bacterial resistance :
Ampicillin
cloning :
backbonepCMV10
backbone_mutationDigested with NotI and BamHI
backbone_origin
backbone_size6281
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Express yeast MCP1 in Expi293F cells
origin :
37
pi :
MCP1shRNA_sequencesize909species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag3xFLAG-PreScission cleavage site

Cloningcloning_site_3
cloning_site
_5promotertd>CMVsequencing_
primer_3agggatgccacccgggatccct
aattcacgtgcaacagcsequ
encing_primer_5aggggccccttgcgg
ccgccATGATAAAGTTGCATGAAGTGCC<
tr>site_3_destroyedtr>site_5_destroyedd>entrez
_gene
><
tr><
td>id
aliasesYOR228C
geneMCP1
854403
<
/td>
genb
ank_ids<
/table><
td>
NC_00114
7.6
YOR228C
mutation
nameYeast
alt_names
MDM10 complementing protein 1
cloning
clone_methodGibson
resistance markers :
5922
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA