pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

catalog :

184335

citations: 1

Reference
Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, et al. Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition. Neuron. 2022;: pubmed publisher

product information

Catalog Number :

184335

Product Name :

pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

article :

doi	10.1016/j.neuron.2022.04.017
id	28225062
pubmed_id	35550065

bacterial resistance :

Ampicillin

cloning :

backbone

pDisplay-AP-CFP-TM

backbone_mutation

EGFP-2A was inserted after the CMV promoter in frame with the signal peptide

backbone_origin

Alice Ting Lab / Addgene_#20861

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BglII
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Nrxn3
id	18191

genbank_ids

mutation

name

Nrxn3-alpha-GPI

shRNA_sequence

size

4179

species

10090

Mus musculus