I212L | Addgene 184304 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

I212L

catalog :

184304

citations: 1

Reference
Ali R, Zahm J, Rosen M. Bound nucleotide can control the dynamic architecture of monomeric actin. Nat Struct Mol Biol. 2022;29:320-328 pubmed publisher

product information

Catalog Number :

184304

Product Name :

I212L

article :

doi	10.1038/s41594-022-00743-5
id	28225055
pubmed_id	35332323

bacterial resistance :

Bleocin (Zeocin)

cloning :

backbone

pPICZ B

backbone_mutation

backbone_origin

Invitrogen

backbone_size

3328

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.

growth strain :

To study dynamics of G-actin using NMR spectroscopy.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	EcoRI
promoter	AOX1
sequencing_primer_3	5'-GCAAATGGCATTCTGACATCC-3'
sequencing_primer_5	5'-GACTGGTTCCAATTGACAAGC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel CG4027, F-ACTIN, F-Actin, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, actomyosin, anon-EST:fe2D2, beta-Actin, beta-actin, beta-actin/Bap47, chrX:5748184. 5748304, cyt5C, f-actin, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486
gene	Act5C
id	31521

genbank_ids

mutation

I212L

name

Act5C

shRNA_sequence

size

1431

species

7227

Drosophila melanogaster