This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Lyso-PTEN(A4)-mCherry
catalog :
184054
citations: 1
Reference
Chen M, Sun T, Zhong Y, Zhou X, Zhang J. A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity. ACS Cent Sci. 2021;7:2009-2020 pubmed publisher
product information
Catalog Number :
184054
Product Name :
pcDNA3-Lyso-PTEN(A4)-mCherry
article :
doi10.1021/acscentsci.1c00919
id28224594
pubmed_id34963894
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5415
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.
origin :
37
pi :
alt_names
LAMP1-PTEN(S380A,T382/383A,S385A)-mCherry
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliases10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
genePTEN
id5728
genbank_ids
mutationSer 380/385 and Thr 382/383 in PTEN mutated to Ala.
nameLyso-PTEN(A4)-mCherry
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagLysosome-associated membrane protein 1 (LAMP1).
locationC terminal on insert
tagmCherry
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA