pcDNA T7-Gnat1 Mut-3UTR | Addgene 184027 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA T7-Gnat1 Mut-3UTR

catalog :

184027

citations: 1

Reference
Matalkah F, Jeong B, Sheridan M, Horstick E, Ramamurthy V, Stoilov P. The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors. Commun Biol. 2022;5:1011 pubmed publisher

product information

Catalog Number :

184027

Product Name :

pcDNA T7-Gnat1 Mut-3UTR

article :

doi	10.1038/s42003-022-03990-w
id	28225013
pubmed_id	36153373

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(+)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: XhoI (destroyed during cloning). Please visit https://www.biorxiv.org/content/10.1101/2022.03.09.483688v1 for bioRxiv preprint.

growth strain :

Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	pcDNA3.1/BGH reverse priming site
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Gnat-1, Ird1, Ird2, Tralpha, irdc, irdr, transducin
gene	Gnat1
id	14685

genbank_ids

mutation

TAG sites in the 3'-UTR are mutated to TGA

name

Gnat1

shRNA_sequence

size

species

10090

Mus musculus