This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPolI_SARS-CoV-2_NLuc
catalog :
183953
product information
Catalog Number :
183953
Product Name :
pPolI_SARS-CoV-2_NLuc
article :
doi10.1101/2021.06.28.450211
id28224997
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepMK-RQ
backbone_mutation
backbone_originInvitrogen
backbone_size2278
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.450211v2 for bioRxiv preprint.
growth strain :
Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterMammalian Pol I
sequencing_primer_3GCTGGCACGACAGGTTTC
sequencing_primer_5GTGAGCGCGACGTAATAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGU280_gp10
geneN
id43740575
aliasesGU280_gp11
geneORF10
id43740576
genbank_ids
NC_045512.2
mutation
nameSARS-CoV-2 minigenome with Nano luciferase
shRNA_sequence
size2552
species
32644
Other
SARS coronavirus 2
tags
plasmid copy :
Invitrogen/GeneArt: The synthetic gene pPolI_SARS2_NLuc was assembled from synthetic oligonucleotides and/or PCR products by Invitrogen/GeneArt
resistance markers :
5906
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA