This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
catalog :
183951
citations: 1
Reference
Brannan K, Chaim I, Marina R, Yee B, Kofman E, Lorenz D, et al. Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Nat Methods. 2021;18:507-519 pubmed publisher
product information
Catalog Number :
183951
Product Name :
pLIX403_control_APOBEC_HA_P2A_mRuby_Cap
ture1
article :
doi10.1038/s41592-021-01128-0
id28222740
pubmed_id33963355
bacterial resistance :
Ampicillin
cloning :
backbonepLIX_403 (Addgene 41395)
backbone_mutationLR insertion of place-holder gene, followed by restriction cloning and Gibson assembly to isolate APOBEC1 and insert Capture1.
backbone_originRoot Lab, Addgene
backbone_size9396
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterTRE promoter, Tet ON
sequencing_primer_3O.PGK1b-R
sequencing_primer_5LNCX
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesREPR, apobec-1
geneApobec1
id25383
genbank_ids
mutation
nameAPOBEC1
shRNA_sequence
size687
species
10116
Rattus norvegicus
tags
resistance markers :
2793
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA