This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
NLS-tdPCP-CIBN
catalog :
183937
citations: 1
Reference
Trojanowski J, Frank L, Rademacher A, Mücke N, Grigaitis P, Rippe K. Transcription activation is enhanced by multivalent interactions independent of phase separation. Mol Cell. 2022;82:1878-1893.e10 pubmed publisher
product information
Catalog Number :
183937
Product Name :
NLS-tdPCP-CIBN
article :
doi10.1016/j.molcel.2022.04.017
id28224994
pubmed_id35537448
bacterial resistance :
Kanamycin
cloning :
backbonetdTomato-N1
backbone_mutation
backbone_originAddgene #54642
backbone_size4592
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
under control of the weakened deltaTATA-CMV promoter for low level expression
growth strain :
PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure
origin :
37
pi :
alt_names
tandem PP7 coat protein
CIB1, N-terminal domain
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5HindIII
promoterCMV (TATA box removed)
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1
geneCIB1
id829604
genbank_ids
mutationnone
nametdPCP-CIBN
shRNA_sequence
size
species
3702
Arabidopsis thaliana
100
Synthetic
tags
locationN terminal on insert
tagNLS
resistance markers :
3654
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA