PHR-GFP-FUSN-VP16 | Addgene 183933 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PHR-GFP-FUSN-VP16

catalog :

183933

citations: 1

Reference
Trojanowski J, Frank L, Rademacher A, Mücke N, Grigaitis P, Rippe K. Transcription activation is enhanced by multivalent interactions independent of phase separation. Mol Cell. 2022;82:1878-1893.e10 pubmed publisher

product information

Catalog Number :

183933

Product Name :

PHR-GFP-FUSN-VP16

article :

doi	10.1016/j.molcel.2022.04.017
id	28224994
pubmed_id	35537448

bacterial resistance :

Kanamycin

cloning :

backbone

pmCherryN1

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

origin :

pi :

alt_names

PHR domain of CRY2

transactivation domain of UL48 (VP16)

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BsrGI
promoter	CMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
gene	CRY2
id	839529

aliases	HHV1gp00p27
gene	UL48
id	24271473

genbank_ids

mutation

none

name

PHR-GFP-FUSN-VP16

shRNA_sequence

size

species

3702

Arabidopsis thaliana

100

Synthetic