This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pX459-HypaCas9-ACTB_sgRNA
catalog :
183885
citations: 1
Reference
Husser M, Ozugergin I, Resta T, Martin V, Piekny A. Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA. Open Biol. 2022;12:220247 pubmed publisher
product information
Catalog Number :
183885
Product Name :
pX459-HypaCas9-ACTB_sgRNA
article :
doi10.1098/rsob.220247
id28224986
pubmed_id36416720
bacterial resistance :
Ampicillin
cloning :
backbonepX459V2.0-HypaCas9
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.
origin :
37
pi :
alt_names
Actin beta
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3ggccatttaccgtaagttatgtaacg
sequencing_primer_5gagggcctatttcccatgattcc
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutation
nameACTB sgRNA spacer
shRNA_sequenceGCCGTTGTCGACGACGAGCG
size
species
9606
Homo sapiens
tags
resistance markers :
5902
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA