This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pODN-mR2-ACTB
catalog :
183868
citations: 1
Reference
Husser M, Ozugergin I, Resta T, Martin V, Piekny A. Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA. Open Biol. 2022;12:220247 pubmed publisher
product information
Catalog Number :
183868
Product Name :
pODN-mR2-ACTB
article :
doi10.1098/rsob.220247
id28224986
pubmed_id36416720
bacterial resistance :
Ampicillin
cloning :
backbonepJET1.2
backbone_mutation
backbone_originThermo Fischer Scientific
backbone_size2974
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
Other
Donor template
growth strain :
Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
origin :
37
pi :
alt_names
Actin beta
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GTTCCTGATGAGGTGGTTAGCATAG
sequencing_primer_5CAACTGCTTTAACACTTGTGCCTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutationHomology arms contain point mutations to remove the sgRNA target site
nameACTB homology arms with mRuby2-linker
shRNA_sequence
size2726
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmRuby2-linker
resistance markers :
5902
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA