This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
NES-EGFP-PH-BTKx2
catalog :
183652
citations: 1
Reference
Walpole G, Pacheco J, Chauhan N, Clark J, Anderson K, Abbas Y, et al. Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase. Nat Cell Biol. 2022;24:708-722 pubmed publisher
product information
Catalog Number :
183652
Product Name :
NES-EGFP-PH-BTKx2
article :
doi10.1038/s41556-022-00895-y
id28224944
pubmed_id35484249
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
PtdIns(3,4,5)P3 biosensor
origin :
37
pi :
alt_names
AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-Fwd, EGFP-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA
geneBTK
id695
genbank_ids
mutationAmino acids 2-174 and 2-170
nameBTK
shRNA_sequence
size
species
9606
Homo sapiens
8355
Xenopus laevis
tags
locationN terminal on insert
tagX. laevis map2k1.L(32-44)-EGFP
resistance markers :
177
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA