pDRM56 tet-AR(1-707)C617Y | Addgene 183504 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDRM56 tet-AR(1-707)C617Y

catalog :

183504

citations: 1

Reference
Hickey T, Selth L, Chia K, Laven Law G, Milioli H, Roden D, et al. The androgen receptor is a tumor suppressor in estrogen receptor-positive breast cancer. Nat Med. 2021;27:310-320 pubmed publisher

product information

Catalog Number :

183504

Product Name :

pDRM56 tet-AR(1-707)C617Y

article :

doi	10.1038/s41591-020-01168-7
id	28224917
pubmed_id	33462444

bacterial resistance :

Ampicillin

cloning :

backbone

pCW57.1

backbone_mutation

backbone_origin

David Root (Addgene plasmid # 41393)

backbone_size

7705

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Pair with pDRM55 (Addgene #183503)for comparative studies. Please note: Plasmid contains a R211A mutation and Gly 466-470 deletion in AR(1-707)C617Y. These differences are not known to affect plasmid function.

growth strain :

Lentiviral vector expressing a doxycycline-inducible truncated DNA-binding mutant androgen receptor (AR mutant C617Y)

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	hPGK R (CCCAGGGCTGCCTTGGAAAAG)
sequencing_primer_5	CMV F (GAGCTCGTTTAGTGAACCGTC)
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

Truncated mutant AR; spans AR 1-707 aa, and contains a C617Y loss-of-function mutation in the DNA binding domain (c.1850 G>A)

name

Androgen receptor

shRNA_sequence

size

2124

species

9606

Homo sapiens