pFUGW-shRII -RII S97E-IRES-EGFP | Addgene 183458 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFUGW-shRII -RII S97E-IRES-EGFP

catalog :

183458

citations: 1

Reference
Church T, Tewatia P, Hannan S, Antunes J, Eriksson O, Smart T, et al. AKAP79 enables calcineurin to directly suppress protein kinase A activity. elife. 2021;10: pubmed publisher

product information

Catalog Number :

183458

Product Name :

pFUGW-shRII -RII S97E-IRES-EGFP

article :

doi	10.7554/eLife.68164
id	28224900
pubmed_id	34612814

bacterial resistance :

Ampicillin

cloning :

backbone

pFUGW-H1 empty vector

backbone_mutation

backbone_origin

pFUGW-H1 empty vector was a gift from Sally Temple (Addgene plasmid # 25870 ; http://n2t.net/addgene:25870 ; RRID:Addgene_25870)

backbone_size

10130

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Zeo marker is outside the LTRs and will not be packaged into virus.

growth strain :

Replacement of endogenous rat PKA-RII subunits with the RII S97E variant

origin :

pi :

alt_names

cAMP-dependent protein kinase type II-alpha regulatory subunit

cloning

clone_method	Restriction Enzyme
cloning_site_3	GOI: NheI
cloning_site_5	GOI: AgeI
promoter	shRNA: H1 / gene: ubiquitin
sequencing_primer_3	5'GTTCAGGGGGAGGTGT
sequencing_primer_5	shRNA (H1) - 5'tcgctatgtgttctgggaaa; Prkar2a_bp500_F - 5'GAGATGATGGAGACAACTTTTATGTC; Prkar2a_bp1000_F - 5'GGAGAACTTGCCCTGGTGACCA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Prkar2a
id	29699

genbank_ids

mutation

shRNA-resistant mutations: C135>G, G138>A, A141>G. Phosphomimetic: S97E

name

sgRNA and Prkar2a

shRNA_sequence

GCCAGGAATCAGACTCGTTCA

size

species

10116

Rattus norvegicus