This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
catalog :
183364
citations: 1
Reference
Devant P, Cao A, Kagan J. Evolution-inspired redesign of the LPS receptor caspase-4 into an interleukin-1β converting enzyme. Sci Immunol. 2021;6: pubmed publisher
product information
Catalog Number :
183364
Product Name :
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
article :
doi10.1126/sciimmunol.abh3567
id28224889
pubmed_id34734155
bacterial resistance :
Ampicillin
cloning :
backbonepMSCV-IRES-EGFP
backbone_mutation
backbone_origin
backbone_size6400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth strain :
Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NotI
promoterMESV
sequencing_primer_3MSCV-rev
sequencing_primer_5MSCV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASP1
geneCASP4
id403724
genbank_ids
mutationH342D_K345M_K379T
nameCaspase-1/4a
shRNA_sequence
size1200
species
32644
Other
Canis lupus familiaris
tags
locationN terminal on insert
tagMyc
resistance markers :
1872
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA