This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLentiCRISPR_zeo_sgTP53
catalog :
183180
citations: 1
Reference
Stransky L, Vigeant S, Huang B, West D, Denize T, Walton E, et al. Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not require an intact p53 pathway. Proc Natl Acad Sci U S A. 2022;119:e2120403119 pubmed publisher
product information
Catalog Number :
183180
Product Name :
pLentiCRISPR_zeo_sgTP53
article :
doi10.1073/pnas.2120403119
id28224849
pubmed_id35357972
bacterial resistance :
Ampicillin
cloning :
backbonepLentiCRISPRv2
backbone_mutationModified to replace PuroR gene with BleoR gene
backbone_origin
backbone_size12767
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth strain :
pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53
origin :
30
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BSMBI
cloning_site_5BSMBI
promoterU6
sequencing_primer_3
sequencing_primer_5U6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation
namesgTP53
shRNA_sequenceCCATTGTTCAATATCGTCCG
size1179
species
9606
Homo sapiens
tags
resistance markers :
238
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA