pLenti_EF1a_Flag_TP53_R248W+P278A | Addgene 183115 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti_EF1a_Flag_TP53_R248W+P278A

catalog :

183115

citations: 1

Reference
Stransky L, Vigeant S, Huang B, West D, Denize T, Walton E, et al. Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not require an intact p53 pathway. Proc Natl Acad Sci U S A. 2022;119:e2120403119 pubmed publisher

product information

Catalog Number :

183115

Product Name :

pLenti_EF1a_Flag_TP53_R248W+P278A

article :

doi	10.1073/pnas.2120403119
id	28224849
pubmed_id	35357972

bacterial resistance :

Kanamycin

cloning :

backbone

pLenti-EF1 -Gate-PGK-hygromycin

backbone_mutation

backbone_origin

backbone_size

10053

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

Please note: plasmid contains the P72R mutation from a common germline SNP in TP53.

growth strain :

Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3	3' PGK-rev: CTTTTGAAGCGTGCAGAATG
sequencing_primer_5	3' EF1a-fwd: CTTCCATTTCAGGTGTCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

changed proline 278 to alanine and arginine 248 to tryptophan, see depositor's comments below

name

TP53

shRNA_sequence

size

1179

species

9606

Homo sapiens