pLenti_EF1a_Flag_TP53_R248W | Addgene 183114 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti_EF1a_Flag_TP53_R248W

catalog :

183114

citations: 1

Reference
Stransky L, Vigeant S, Huang B, West D, Denize T, Walton E, et al. Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not require an intact p53 pathway. Proc Natl Acad Sci U S A. 2022;119:e2120403119 pubmed publisher

product information

Catalog Number :

183114

Product Name :

pLenti_EF1a_Flag_TP53_R248W

article :

doi	10.1073/pnas.2120403119
id	28224849
pubmed_id	35357972

bacterial resistance :

Kanamycin

cloning :

backbone

pLenti-EF1 -Gate-PGK-hygromycin

backbone_mutation

backbone_origin

Gift

backbone_size

10053

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth strain :

Expresses flag-tagged p53 with R248W mutation in mammalian cells

origin :

pi :

alt_names

LFS1, Cellular Tumor Antigen P53

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3	3' PGK-rev: CTTTTGAAGCGTGCAGAATG
sequencing_primer_5	3' EF1a-fwd: CTTCCATTTCAGGTGTCGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

changed arginine 248 to tryptophan

name

TP53

shRNA_sequence

size

1179

species

9606

Homo sapiens