This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBY011-AMN1ko
catalog :
183099
citations: 1
Reference
Guinn L, Lo E, BALAZSI G. Drug-dependent growth curve reshaping reveals mechanisms of antifungal resistance in Saccharomyces cerevisiae. Commun Biol. 2022;5:292 pubmed publisher
product information
Catalog Number :
183099
Product Name :
pBY011-AMN1ko
article :
doi10.1038/s42003-022-03228-9
id28224847
pubmed_id35361876
bacterial resistance :
Ampicillin
cloning :
backbonepBY011
backbone_mutationNone
backbone_originHarvard Medical School
backbone_size3607
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
growth strain :
S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
origin :
37
pi :
alt_names
AMN1 (YBR158W)
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterURA3, TEF1
sequencing_primer_3AACACTATGAGTGTATCTTTTGGAG
sequencing_primer_5GAACTAACAAAAACGTTCAAAAAGTTT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBR158W, CST13, ICS4
geneAMN1
id852455
genbank_ids
EEU05423
EEU05423.1
mutationonly includes external sequences (504 bp each) as homology arms, separated by KanMX
nameAMN1 (left homology arm) - KanMX - AMN1 (right homology arm)
shRNA_sequence
size2365
species
4932
Saccharomyces cerevisiae
tags
location
tagKanMX
resistance markers :
1506
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA