This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb
catalog :
183078
citations: 1
Reference
Hodis E, Torlai Triglia E, Kwon J, Biancalani T, Zakka L, Parkar S, et al. Stepwise-edited, human melanoma models reveal mutations' effect on tumor and microenvironment. Science. 2022;376:eabi8175 pubmed publisher
product information
Catalog Number :
183078
Product Name :
pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb
article :
doi10.1126/science.abi8175
id28224839
pubmed_id35482859
bacterial resistance :
Ampicillin
cloning :
backboneAAV
backbone_mutationReplaced all sequence between AAV ITRs with insert acceptance site and PGK-Puro-PolyA.
backbone_originReceived from R. Platt from F. Zhang lab, Broad Institute
backbone_size4153
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
Other
Homologous Recombination Donor Template
growth strain :
rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
origin :
37
pi :
alt_names
BRAF Exon 15
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5MluI
promoterNone for primary insert. PGK for puromycin resistance gene.
sequencing_primer_3NGS
sequencing_primer_5NGS
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutationV600E (T>A), S607S (TCC>AGT)
nameBRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms
shRNA_sequence
size1837
species
9606
Homo sapiens
tags
resistance markers :
3145
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA