This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
catalog :
182921
citations: 1
Reference
Cuylen Haering S, Petrovic M, Hernandez Armendariz A, Schneider M, Samwer M, Blaukopf C, et al. Chromosome clustering by Ki-67 excludes cytoplasm during nuclear assembly. Nature. 2020;587:285-290 pubmed publisher
product information
Catalog Number :
182921
Product Name :
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
article :
doi10.1038/s41586-020-2672-3
id28224783
pubmed_id32879492
bacterial resistance :
Ampicillin
cloning :
backbonepIRESpuro2
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3ATTCCAGCACACTGGATCA
sequencing_primer_5CAGAGCTGGTTTAGTGAACC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesKIA, MIB-, MIB-1, PPP1R105
geneMKI67
id4288
genbank_ids
mutationRASA mutation
nameMKI67
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
locationC terminal on backbone
tagmCherry
resistance markers :
162
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA