This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-2T-TRR-C421-G2304D
catalog :
182845
citations: 1
Reference
Tie F, Banerjee R, Saiakhova A, Howard B, Monteith K, Scacheri P, et al. Trithorax monomethylates histone H3K4 and interacts directly with CBP to promote H3K27 acetylation and antagonize Polycomb silencing. Development. 2014;141:1129-39 pubmed publisher
product information
Catalog Number :
182845
Product Name :
pGEX-2T-TRR-C421-G2304D
article :
doi10.1242/dev.102392
id28224770
pubmed_id24550119
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-2T
backbone_mutationadd NdeI and NsiI sites between BamHI and EcoRI sites
backbone_origin
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The cDNA clones of TRR (SD13650) was obtained from the Drosophila Genomics Resource Center (DGRC). Additional mutation was found at Q2050L. This is outside of the TRR SET domain (2293-2431 aa) and has no effect on its enzyme activity.
growth strain :
mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites
origin :
37
pi :
alt_names
trithorax-related
cloning
clone_methodRestriction Enzyme
cloning_site_3NsiI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5atgggtgcaggagcaggcgccac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG3848, CG3848, Dmel CG3848, EG:63B12.3, TRR, Trr, anon-2Bb, dmll3/4
genetrr
id31149
genbank_ids
mutationmutation G2304D (GGC/GAC)
nameTRR 2011-2431
shRNA_sequence
size1263
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagGST
resistance markers :
5853
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA