This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT-FLAG-CBP (N+HAT)
catalog :
182841
citations: 1
Reference
Tie F, Banerjee R, Stratton C, Prasad Sinha J, Stepanik V, Zlobin A, et al. CBP-mediated acetylation of histone H3 lysine 27 antagonizes Drosophila Polycomb silencing. Development. 2009;136:3131-41 pubmed publisher
product information
Catalog Number :
182841
Product Name :
pMT-FLAG-CBP (N+HAT)
article :
doi10.1242/dev.037127
id28224766
pubmed_id19700617
bacterial resistance :
Ampicillin
cloning :
backbonepMT
backbone_mutationFLAG tag at N-terminus before NheI site
backbone_origin
backbone_size3850
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBP N. Expression in S2 cells by CuSO4 induction.
origin :
37
pi :
alt_names
nej
cloning
clone_methodRestriction Enzyme
cloning_site_3NsiI
cloning_site_5NheI
promoterMT
sequencing_primer_3
sequencing_primer_5gctagcaatccactg ctcct
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]]
genew
id31271
genbank_ids
mutationdletion of residues 1566-2142 from CBP1476-3222
nameCBP
shRNA_sequence
size3510
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagFLAG tag
plasmid copy :
The CBP cDNA clone was from Sarah Smolik
resistance markers :
5853
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA