This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pORANGE_Tubb3 GFP(Y39TAG) KI
catalog :
182678
citations: 1
Reference
Arsic A, Hagemann C, Stajković N, Schubert T, Nikić Spiegel I. Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat Commun. 2022;13:314 pubmed publisher
product information
Catalog Number :
182678
Product Name :
pORANGE_Tubb3 GFP(Y39TAG) KI
article :
doi10.1038/s41467-022-27956-y
id28224672
pubmed_id35031604
bacterial resistance :
Ampicillin
cloning :
backbonepORANGE
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
origin :
37
pi :
alt_names
Tubb3
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterU6 and chicken beta-actin promoter
sequencing_primer_3cgggccatttaccgtaagtt
sequencing_primer_5aaggctgttagagagataattggaa
site_3_destroyed
site_5_destroyed
entrez_gene
aliases3200002H15Rik, M(beta)3, M(beta)6
geneTubb3
id22152
aliases
geneTubb3
id246118
genbank_ids
NM_023279.3
NM_139254.2
mutation
nameTubb3-targeting gRNA and GFP(Y39TAG) donor sequence
shRNA_sequenceGCTGCGAGCAACTTCACTT
size
species
10090
Mus musculus
10116
Rattus norvegicus
tags
plasmid copy :
This plasmid is based on the pORANGE Tubb3-GFP KI plasmid: https://www.addgene.org/131497/. We replaced WT GFP with the GFP(Y39TAG), all other sequences were unchanged.
resistance markers :
5928
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA