This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTRIP TRE-BI GFP10-RHOA/RBD-GFP11
catalog :
182232
citations: 1
Reference
Koraïchi F, Gence R, Bouchenot C, Grosjean S, Lajoie Mazenc I, Favre G, et al. High-content tripartite split-GFP cell-based assays to screen for modulators of small GTPase activation. J Cell Sci. 2018;131: pubmed publisher
product information
Catalog Number :
182232
Product Name :
pTRIP TRE-BI GFP10-RHOA/RBD-GFP11
article :
doi10.1242/jcs.210419
id28193257
pubmed_id29192060
bacterial resistance :
Ampicillin
cloning :
backbonepTRIP TRE
backbone_mutation
backbone_origin
backbone_size10000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5SacII
promoterTRE bidirectional
sequencing_primer_3gtggctaagatctacagctg
sequencing_primer_5ATTTCGAGCTCGGTACCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
NM 001313944
mutation
nameras homolog family member A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP10 (split-GFP tripartite)
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5NdeI
promoterTRE bidirectional
sequencing_primer_3ggacagcagagatccactt
sequencing_primer_5CTGGAGAATTCACCGGTCATATG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
NM_001136227.1
mutation
namerhotekin RHO binding domain (RBD)
shRNA_sequence
size252
species
10090
Mus musculus
tags
locationC terminal on insert
tagGFP11 (M4)
resistance markers :
4642
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA