This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR
catalog :
181974
citations: 1
Reference
Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman J, Xue X, et al. A genome-scale screen for synthetic drivers of T cell proliferation. Nature. 2022;603:728-735 pubmed publisher
product information
Catalog Number :
181974
Product Name :
pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR
article :
doi10.1038/s41586-022-04494-7
id28224616
pubmed_id35296855
bacterial resistance :
Ampicillin
cloning :
backbonelentiCRISPR v2
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
geneNGFR
id4804
genbank_ids
mutationNGFR truncation: aa 11-276
nameFMC6.3-28z CAR; tNGFR
shRNA_sequence
size
species
9606
Homo sapiens
100
Synthetic
tags
resistance markers :
4810
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA