This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-GFP11(x7)-Actin
catalog :
181967
citations: 1
Reference
Tenner B, Zhang J, Kwon Y, Pessino V, Feng S, Huang B, et al. FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains. Sci Adv. 2021;7: pubmed publisher
product information
Catalog Number :
181967
Product Name :
pcDNA3-GFP11(x7)-Actin
article :
doi10.1126/sciadv.abe4091
id28219895
pubmed_id34020947
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5510
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Human beta actin tagged with 7 copies of GFP11.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
mutation
nameGFP11(x7)-Actin
shRNA_sequence
size1818
species
tags
locationN terminal on backbone
tag6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
locationN terminal on insert
tagGFP11(x7)
plasmid copy :
DNA encoding the GFP11(x7) array was provided by Bo Huang (UCSF).
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA